Eintracht Frankfurt will play Arsenal at the Emirates Stadium later this month without a single fan.
This follows a ban by UEFA on the club following crowd trouble during a Europa League match against Vitoria in Portugal, where they threw objects leading to a late kickoff.
The club had lodged an appeal with the governing body, but it was thrown out as per the club's communication on Thursday.
Last season's semi-finalists were already suspended from previous breaches, and in addition to the Arsenal match, the team will also serve another ban in a European fixture.
"The appeal of Eintracht Frankfurt against the judgment of the UEFA Control, Ethics and Disciplinary Commission of 17 October 2019 was rejected by the competent chamber last night," a club statement read.
"As a result, the 'cut-off penalty' for the game at Arsenal FC on 28 November in London continues."
Arsenal lead Group F on 10 points, four ahead of their next opponents, whom they beat 3-0 in the group opening match in September.
The Gunners will pick first spot with a win against the Germans, who will be eager to make amends after a poor start to the campaign in Europe.
They still have a chance of making to the next round, but must go battle Standard Liege, who sit second on the log.