Barcelona coach Quique Setién has named a 23-man squad for their match against Leganes on Tuesday night.
It will be the Catalan side's first game at Camp Nou since the league restart, following a three-month break as a result of the coronavirus pandemic.
They could open a five-point gap between them and the closest challengers and archrivals Real Madrid, who will be in action on Thursday if they pick maximum points.
The only change in the squad is the inclusion of defender Clément Lenglet, who missed the 4-0 win over Mallorca on Saturday night through suspension.
They will however the services of Jordi Alba, who is out through suspension, as well as Ousmane Dembélé, who is sidelined with an injury.
In the meantime, the team conducted their last training at the Ciutat Esportiva on Monday, with Messi, Jordi Alba, and De Jong all undergoing the regulatory anti-doping controls administered by the Spanish Anti-Doping Agency.
Squad to face Leganes
Ter Stegen, N. Semedo, Piqué, I. Rakitic, Sergio, Arthur, Suárez, Messi, Neto, Lenglet, Griezmann, Braithwaite, S. Roberto, F. De Jong, Vidal, Umtiti, Junior, Iñaki Peña, Riqui Puig, Collado, Ansu Fati, R. Araujo and Monchu.